Technical Information
Steinle’s Multidrop™ & BioTek™ Cassettes
Lifetime of cassettes
There are different factors to consider, which can influence the EOL:
- Proper maintenance (cleaning, autoclaving, drying, etc.).
- Keeping reagents, cassettes, and the environment free from particles and dust which can lead to clogs.
- Having the cassettes under tension when not in use (better use the rest position).
- Dispensing different reagents with one cassette (better dedicate one cassette to each liquid).
- Dispensing reagents with properties that differ from water (ethanol, DMSO, glycerol).
- Proper recalibration of the cassette.
- Having a low, medium, or high throughput.
- It is recommended to use cassettes within 1 year, securing an accurate calibration.
Handling Tips & Troubleshooting
For special handling tips and troubleshooting of Steinle’s Dispensing Cassettes, click here
For an individual support of your problem, please get in touch.
Lifetime of Steinle’s Multidrop™ Cassettes
Lifetime of cassettes related to total dispensing volume.
Numbers are meant as guidelines, dispensing aqueous solutions.
| Version | Total Volume | Volume Range |
|---|---|---|
| Standard Tube Dispensing Cassette | 6000 x 96 well-plates with 50µ | 5µl-2500µ Best CV and accuracy at 100µ |
| Small Tube Dispensing Cassette | 150 x 1536 well-plates with 10µ | 0.5µl-50µ Best CV and accuracy at 10µ. |
Lifetime of Steinle’s BioTek™ Cassettes
Lifetime of cassettes related to total dispensing volume.
Numbers are meant as guidelines, dispensing aqueous solutions.
| Version | Total Volume | Volume Range |
|---|---|---|
| 1µ-Low Volume | 1,000x 384-well microplates at 5 μL per well | 1 μL to 50 μL Best CV and accuracy at >2µ |
| 5µ-Medium Volume | 1,000x 96-well microplates at 50 μL per well | 5 μL to 2,500 μL Best CV and accuracy at >10µ. |
| 10µ-Large Volume | 1,000x 96-well microplates at 100 μL per well | 10 μL to 3,000 μL Best CV and accuracy at >10µ. |
Accuracy & Precision
For Steinle’s Multidrop™ Dispensing Cassettes
| Value | Small Tube Dispensing Cassette | Standard Tube Dispensing Cassette |
| Dispensing precision (CV%) | 0.5 μl: CV≤ 8% 2.0 μl: CV≤ 5% 10 μl: CV≤ 3% >10 μl: CV≤ 3% | 5 μl: CV≤ 10% 20 μl: CV≤ 1.5% 100 μl: CV≤ 1% >100 μl: CV≤ 1% |
| Dispensing accuracy | 2.0 μl: ± 8% (typical) 10 μl: ± 5% (typical) > 10 μl: ± 5% (typical) | 5 μl: ± 3% (typical) 20 μl: ± 2% (typical) 100 μl: ± 1% (typical) > 100 μl: ± 1% (typical) |
Accuracy & Precision
For Steinle’s BioTek™ Dispense Cassettes
| Value | 1 μl – Low Volume | 5 µl – Medium Volume | 10 µl – Large Volume |
| Dispensing precision (CV%) | 10% @ 1 μL 5% @ >=2 μL | 5% @ 5 μL 2.5% @ ³10 μL | 4% @ 10 μL 2% @ >=20 μL |
| Dispensing accuracy | ± 10% @ 1 μL ± 5% @ >=2 μL | ± 4% @ 5 μL ± 2%@ >=10 μL | ± 4% @ 10 μL ± 2% @ >=20 μL |
Additional Information
Material
Dispensing Cassettes from Steinle Labtechnology use the same materials as conventional cassettes.
Materials that get in contact with liquids are:
Multidrop®:
Polypropylene and silicone for all Standard and Small Tube plastic tipped versions .
Stainless steel, ruby and silicone for all Small Tube Metal tipped versions.
Stainless steel and silicone for the Standard Tube Metal Tip version.
BioTek®:
Polypropylene and silicone for all plastic tipped versions .
Stainless steel, ruby and silicone for all Metal Tip, ruby orifice versions.
Stainless steel and silicone for all Metal Tip versions.
Warranty
Dispensing cassettes are consumables and therefore only have a limited warranty in case of malfunction.
The warranty is valid from the date you received the product, till 90 days after.
Repair or substitution is guaranteed through Steinle Labtechnology after receipt of dysfunctional product(s).
The warranty isn’t valid in case of product misuse or unusual and destructive treatment.
Calibration
All Steinle Cassettes come calibrated and ready to use including a calibration report.
A recalibration should be performed once a month or if left under tension for a prolonged period.
Autoclavability
Standard tube: 50 times (1 bar pressure at 121°C for 20 minutes).
Small tube: 10 times (1 bar pressure at 121°C for 20 minutes)
Origin of Products
Steinle Labtechnology independently develops and manufactures exclusive and compatible versions for Multidrop® & BioTek® Dispenser.
All products are originated quality made in Germany.
Multidrop® is a registered trademark of Thermo Fisher Scientific.
BioTek® is a registered trademark of BioTek Instruments, Inc.
Liquid compliance
List of dispensable liquids with all Steinle Cassettes.
If you have questions on how to deal best with a specific media, please get in touch and we will assist you to our best knowledge.
- Buffers
- Hanks Hepes, cells. Cis HTRF kit
- IMAP detection reagent
- IMAP 1x kinase buffer
- 50nM HEPES, 10% glycerol, 0.1 % BSA
- TempliPhi reagent
- PBS buffer
- Tris buffer
- 0.1% Brij35 buffer
- 100 mM sodium phosphate w/ BSA for enzyme
- 20% Tris base
- 145 mM HEPES, 0.1% NaCl
- Organic Solvents
- Acetone
- Acetonitirile
- DMSO, dimethyl sulfoxide
- Ethanol
- Isopropanol
- Viscose Solutions 10% Glycerol
- Acetic acid
- 0.1% Brij35 buffer
- 100 mM sodium phosphate w/ BSA for enzyme
- 20% Tris base
- 145 mM HEPES, 0.1% NaCl
- Growth Media
- DMEM with 10% FCS
- Kaighn’s media with 10% FBS
- Schneider medium with 10% FBS
- M3 media with and without 10% FCS
- TempliPhi reagent
- PBS buffer
- Tris buffer
- 0.1% Brij35 buffer
- 100 mM sodium phosphate w/ BSA for enzyme
- 20% Tris base
- 145 mM HEPES, 0.1% NaCl
- Beads
- Polystyrene beads
- Alpha screen beads
- Tetra ethylene glycol carboxylated beads (0.8µm)
- Yittrium oxide beads
- TempliPhi reagent
- PBS buffer
- Tris buffer
- 0.1% Brij35 buffer
- 100 mM sodium phosphate w/ BSA for enzyme
- 20% Tris base
- 145 mM HEPES, 0.1% NaCl
- Protein Solution
- 0.1% BSA
- Plasma
- Agar
- 50nM HEPES, 10% glycerol, 0.1 % BSA
- TempliPhi reagent
- PBS buffer
- Tris buffer
- 0.1% Brij35 buffer
- 100 mM sodium phosphate w/ BSA for enzyme
- 20% Tris base
- 145 mM HEPES, 0.1% NaCl
